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The testes and sperm of the mouse contain a-actinin, a cytoplasmic actin-binding protein that may be involved in nuclear transport. Here we describe the purification and the sequence of mouse a-actinin. The protein was purified from the testes of a mature mouse by ammonium sulfate precipitation and chromatography on DEAE-Sepharose, Q-Sepharose, and Sephacryl S-200. A-Actinin was then isolated by affinity chromatography on agarose-actin, and the active polypeptide was isolated by chromatography on DEAE-Sepharose. Purified a-actinin contained a single polypept
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By use of a monoclonal gluten antibody it was possible to identify in 2 of 18 patients with dermatitis herpetiformis (DH) a high proportion of circulating autoantibodies to gluten (57 and 71% respectively). In both cases, the lesions and the clinical response to gluten withdrawal correlated with the presence of such autoantibodies. In addition, in one case, this autoantibody pattern was associated with abnormal responses to gluten-reactive T cells. The presence of cutaneous lesions in a patient with clinical and serological features of gluten sensitivity indicates a dual trigger to gut and skin in DH. In addition, the autoimmune mechanisms involved in DH suggest that gluten-sensitive patients with this syndrome would be candidates for gluten immunotherapy.Pure problem
By Dexter Findlay
Published: 2003.05.21 05:36 AM
It’s a good thing that they have a creative person on board to write those scripts, because this new Rizzoli & Isles TV show seems to have every classic whodunit crime going on.
The pilot opens with a lawyer (Tony Shalhoub) in the midst of a tough courtroom case with a client who’s crying. The lawyer’s not too keen about the possibility of his next case being based on the very emotional subject of the tears. He drives home and the next scene has a city bus, played by a great rock band that looks like it was looted by the legendary Hunx and His Punx.
After more detective work and a scene about a bank robbery, things seem to be looking up. It’s all going good, until the next story opens — and it’s all about bloody fingerprints on the window of a bus, proving that it was the scene of a bloody crime.
The pilot has a great cast, including Jennifer Finnigan and Theo Rossi, and a great crew. It’s hard to judge the show until it comes on the air, and I think the episodes that air this season could make a great series. If they could keep the show from falling into the whodunit trap, it could be really good.